Indische Wissenschaftler identifizieren antivirale Verbindungen im Kuhurin, die gegen das Chikungunya-Virus wirksam sind

RFK is licking his lips

Link to the study:

[https://pubs.acs.org/doi/abs/10.1021/acsagscitech.5c00786](https://pubs.acs.org/doi/abs/10.1021/acsagscitech.5c00786)

Abstract

„This study investigates the antiviral properties of cow urine distillate (CUD) and identifies compounds with therapeutic potential against Chikungunya virus (CHIKV). Crude CUD was evaluated for antiviral activity using *in vitro* assays, followed by identification of metabolites via GC–MS characterization. Computational docking studies elucidated the binding affinities and critical interactions of these compounds with the CHIKV nsP2 protease (nsP2^(Pro)). Selected compounds were further assessed for inhibition against nsP2^(Pro) using biochemical and cell-based assays. Furthermore, the synergistic effects of CUD combined with natural compounds thymoquinone (TQ) and piperine (PIP) were evaluated against CHIKV replication. Treatment with 2% v/v CUD resulted in ∼90% reduction in CHIKV plaques. Additionally, three of the compounds identified via GC–MS demonstrated substantial inhibition at nontoxic doses, as validated by the FRET assay and the CHIKV plaque-reduction assay. The combination of TQ + PIP + CUD was found to be synergistic, with the combination of 20 μM TQ + 20 μM PIP + 2% CUD exhibiting a 99.85% reduction in CHIKV titer. The present findings establish the antiviral potential of CUD and provide an effective natural remedy against CHIKV infection.“